| 产品英文名称 | Anti-Lamin B1 Monoclonal Antibody (15T1) |
| 免疫原 | 重组蛋白 |
| 宿主 | 小鼠 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | IF, IHC-P, IP, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB 1:2000-5000，IHC：1:50-300，IF：1:200，IP 1:200。 |
| 克隆性 | 单克隆 |
| 纯化工艺 | 通过使用特异性免疫原的亲和层析，从小鼠腹水中纯化抗体。 |
| 产品形式 | 液体溶液 |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of LMNB1 is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. |
| 基因ID | 4001 |
| 别名 | LMNB1; LMN2; LMNB; Lamin-B1 |
| 蛋白质ID | P20700 |
Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.
Fig.2 Immunofluorescence analysis of human lung cancer tissue. 1, Lamin B1 Monoclonal Antibody (15T1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Lamin B1 Monoclonal Antibody (15T1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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