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CK7 单克隆抗体

CK7 Monoclonal Antibody

浏览次数(52) 文献引用(0) 货号(ABM0018)
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商品信息

产品英文名称 CK7 Monoclonal Antibody
免疫原 合成多肽
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:1000-1:2000,IF:1:50-1:200,IHC-P:1:200,IP:1:200。
克隆性 单克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
分子量 54 KD
储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 keratin 7 encoded by KRT7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
基因ID 3855
别名 KRT7; SCL; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
其它 该抗体可检测内源蛋白
蛋白质ID P08729

图片及说明

Fig.1. IHC-P staining of human lung cancer tissue, diluted at 1:200.

Fig.1. IHC-P staining of human lung cancer tissue, diluted at 1:200.

Fig.2. Western blot analysis of 1) Hela, 2) mouse kidney, 3) mouse brain, diluted at 1:2000.

Fig.2. Western blot analysis of 1) Hela, 2) mouse kidney, 3) mouse brain, diluted at 1:2000.

Fig.3. Immunofluorescence analysis of human liver tissue. 1, CK7 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of human liver tissue. 1, CK7 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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