| 产品英文名称 | XRCC4 Monoclonal Antibody |
| 免疫原 | 合成多肽 |
| 宿主 | 小鼠 |
| 反应性 | 人 |
| 应用 | IF, IHC-P, IP, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:2000，IF：1:100-1:200，IHC-P：1:50-1:300，IP：1:200。 |
| 克隆性 | 单克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从小鼠腹水中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 分子量 | 38 KD |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | X-ray repair cross complementing 4 encoded by XRCC4 functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. X-ray repair cross complementing 4 plays a role in both non-homologous end joining and the completion of V (D)J recombination. Mutations in XRCC4 can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. |
| 基因ID | 7518 |
| 别名 | XRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4 |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | Q13426 |
Fig.1. Western blot analysis of 1) Hela, 2) 293T, diluted at 1:3000.
Fig.2. Immunofluorescence analysis of human liver cancer tissue. 1, XRCC4 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, XRCC4 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
微信公众号
评价
目前还没有评论