| 产品英文名称 | β I tubulin Monoclonal Antibody |
| 免疫原 | 合成多肽 |
| 宿主 | 小鼠 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | IF, IHC-P, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:5000-1:10000，IF：1:200，IHC-P：1:50-300。 |
| 克隆性 | 单克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从小鼠腹水中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | TUBB1 encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. tubulin beta 1 class VI is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in TUBB1 is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of tTUBB1 are found on chromosome Y. |
| 基因ID | 81027 |
| 别名 | TUBB1; Tubulin beta-1 chain; TUBB2A; TUBB2; Tubulin beta-2A chain; TUBB2B; Tubulin beta-2B chain; TUBB4B; TUBB2C; Tubulin beta-4B chain; Tubulin beta-2 chain; Tubulin beta-2C chain; TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-II |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | Q9H4B7 |
Fig.1. Western blot analysis of 1) Hela, 2) mouse brain tissue, 3) rat brain tissue, diluted at 1:5000.
Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, β I tubulin Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, β I tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, β I tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, β I tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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