产品英文名称 | CD15 Monoclonal Antibody |
免疫原 | 合成多肽 |
宿主 | 小鼠 |
反应性 | 人 |
应用 | IF, IHC-P |
实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:IF:1:100-1:200,IHC-P:1:100。 |
克隆性 | 单克隆 |
纯化工艺 | 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。n |
产品形式 | 液体溶液 |
浓度 | 1 mg/ml |
储存缓冲液 | PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
背景 | The product of FUT4 transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). |
基因ID | 2526 |
别名 | FUT4; ELFT; FCT3A; Alpha-(1,3)-fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
其它 | 该抗体可检测内源蛋白 |
蛋白质ID | P22083 |
Fig.1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, CD15 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.2. Immunofluorescence analysis of human liver cancer tissue. 1, CD15 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using CD 15 Mouse mAb diluted at 1:500.
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