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ε微管蛋白小鼠单克隆抗体 (3G1)

Epsilon Tubulin Mouse Monoclonal Antibody (3G1)

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商品信息

产品英文名称 Epsilon Tubulin Mouse Monoclonal Antibody (3G1)
免疫原 合成多肽: Epsilon Tubulin
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:IHC-p:1:100-200。
克隆性 单克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 TUBE1 (tubulin epsilon 1) encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of TUBE1 is found on chromosome 5.
基因ID 51175
别名 Tubulin epsilon chain (Epsilon-tubulin)
其它 该抗体可检测内源蛋白
蛋白质ID Q9UJT0

图片及说明

Fig.1. Immunofluorescence analysis of human stomach tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.1. Immunofluorescence analysis of human stomach tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of mouse spleen tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of mouse spleen tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Epsilon Tubulin Mouse Monoclonal Antibody (3G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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