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Cleaved-Caspase-3 p17 (D175)多克隆抗体

Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody

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商品信息

产品英文名称 Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody
免疫原 合成多肽:人源蛋白的中央区域,Caspase-3 p17,AA:100-180
宿主
反应性 人, 大鼠, 小鼠
应用 ELISA, IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IF:1:50-1:300,IHC-P:1:50-1:300,ELISA:1:20000。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
分子量 20 KD
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 CASP3 encodes caspase 3 which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 3 cleaves and activates caspases 6, 7 and 9, and caspase 3 itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.
基因ID 836
别名 CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
其它 该抗体可检测内源蛋白
蛋白质ID P42574

图片及说明

Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieva l (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieva l (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunofluorescence analysis of human lung cancer tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunofluorescence analysis of human lung cancer tissue. 1, Cleaved-Caspase-3 p17 (D175) 多克隆 Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Western Blot analysis of Hela (1, mouse samples (2, rat heart (3, yeast protein (4, rat lung (5, rat brain (6, mouse colon (7, mouse kidney (8, diluted at 1:5000.

Fig.5. Western Blot analysis of Hela (1, mouse samples (2, rat heart (3, yeast protein (4, rat lung (5, rat brain (6, mouse colon (7, mouse kidney (8, diluted at 1:5000.

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