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Cleaved-Caspase-8 (D384)多克隆抗体

Cleaved-Caspase-8 (D384) Polyclonal Antibody

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商品信息

产品英文名称 Cleaved-Caspase-8 (D384) Polyclonal Antibody
免疫原 合成多肽:人源蛋白的C末端,Caspase-8,AA:310-390
宿主
反应性
应用 ELISA, IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IF:1:50-1:300,IHC-P:1:50-1:300,ELISA:1:20000。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
分子量 47, 55 KD
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 CASP8 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
基因ID 841
别名 CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
其它 该抗体可检测内源蛋白
蛋白质ID Q14790

图片及说明

Fig.1. Western Blot analysis of various cells using Cleaved-Caspase-8 (D384) 多克隆 Antibody.

Fig.1. Western Blot analysis of various cells using Cleaved-Caspase-8 (D384) 多克隆 Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast tissue. 1, Cleaved-Caspase-8 (D384) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast tissue. 1, Cleaved-Caspase-8 (D384) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunofluorescence analysis of human breast cancer tissue. 1, Cleaved-Caspase-8 (D384) 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3 , Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of human breast cancer tissue. 1, Cleaved-Caspase-8 (D384) 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3 , Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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