| 产品英文名称 | Stat1 (phospho Tyr701) Polyclonal Antibody |
| 免疫原 | 合成多肽：人源Stat1 磷酸化位点为中心：Y701 |
| 宿主 | 兔 |
| 反应性 | 人, 大鼠, 小鼠, 猴 |
| 应用 | ELISA, IF, IHC-P, IP, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IF：1:50-1:200，IHC-P：1:100-1:300，IP：2-5 ug/mg lysate，ELISA：1:10000。 |
| 克隆性 | 多克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 分子量 | 87 KD |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Signal transducer and activator of transcription 1 encoded by STAT1 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Signal transducer and activator of transcription 1can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. Signal transducer and activator of transcription 1 mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
| 基因ID | 6772 |
| 别名 | STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84 |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P42224 |
Fig.1. Western Blot analysis of mouse brain (1， SY-SY5X (2， diluted at 1:1000.
Fig.2. Immunohistochemical analysis of paraffin-embedded mouse liver tissue. 1, Stat1 (phospho Tyr701) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Stat1 (phospho Tyr701) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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