加入收藏 欢迎光临Abbkine Scientific Co., Ltd官方网站
登录 注册
中文
0

购物车

¥0

Stat3 (磷酸化-Ser727)多克隆抗体

Stat3 (phospho Ser727) Polyclonal Antibody

浏览次数(23) 文献引用(0) 货号(ABP0046)
  • 产品概述
  • FAQ
  • 文献引用(0)
  • 用户评论(0)

商品信息

产品英文名称 Stat3 (phospho Ser727) Polyclonal Antibody
免疫原 合成多肽:人源Stat3 磷酸化位点为中心:S727
宿主
反应性 人, 大鼠, 小鼠, 猴
应用 ELISA, IF, IHC-P, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IF:1:50-1:200,IHC-P:1:100-1:300,IP:2-5 ug/mg lysate,ELISA:1:10000。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
分子量 85 KD
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Signal transducer and activator of transcription 3 encoded by STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Signal transducer and activator of transcription 3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. Signal transducer and activator of transcription 3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in STAT3 are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
基因ID 6774
别名 STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
其它 该抗体可检测内源蛋白
蛋白质ID P40763

图片及说明

Fig.1. Western Blot analysis of various cells using Phospho-Stat3 (S727) 多克隆 Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using Phospho-Stat3 (S727) 多克隆 Antibody diluted at 1:2000.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Stat3 (phospho Ser727) 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

本产品已在0种出版物中被引用

评价

目前还没有评论

成为第一个评论 “Stat3 (磷酸化-Ser727)多克隆抗体” 的人

邮箱地址不会被公开。 必填项已用*标注

4 + 1 =

意见反馈
在线咨询
扫码关注

微信公众号

返回顶部