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磷酸甘油醛脱氢酶多克隆抗体

GAPDH Polyclonal Antibody

浏览次数(27) 文献引用(0) 货号(ABP50163)
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商品信息

产品英文名称 GAPDH Polyclonal Antibody
免疫原 重组蛋白
宿主
反应性 斑马鱼
应用 WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:2000-5000。
克隆性 多克隆
纯化工艺 使用特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. Glyceraldehyde-3-phosphate dehydrogenase has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Glyceraldehyde-3-phosphate dehydrogenase has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, Glyceraldehyde-3-phosphate dehydrogenase contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
基因ID 2597
别名 GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
其它 该抗体可检测内源蛋白
蛋白质ID P04406

图片及说明

Fig. Western blot analysis of Zebrafish skeletal muscle, diluted at 1:5000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of Zebrafish skeletal muscle, diluted at 1:5000. Secondary antibody was diluted at 1:20000.

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