肌动蛋白β多克隆抗体

Actin β Polyclonal Antibody

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商品信息

产品英文名称	Actin β Polyclonal Antibody
免疫原	合成多肽：来源于N端的人源蛋白Actin β.
宿主	兔
反应性	人, 大鼠, 小鼠
应用	ELISA, IF, IHC-P, WB
实验建议	最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:1000-1:4000，IHC-p：1:100-1:300，ELISA：1:20000。尚未在其他应用中测试。
克隆性	多克隆
纯化工艺	使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。

商品属性

产品形式	液体溶液
浓度	1 mg/ml
储存缓冲液	含有50％甘油，0.5％BSA和0.02％叠氮化钠的PBS缓冲液。
保存建议	自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件	蓝冰运输
警告	本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

附加信息

背景	ACTB encodes one of six different actin proteins (actin beta). Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
基因ID	60
别名	ACTB; Actin; cytoplasmic 1; Beta-actin
其它	该抗体可检测内源蛋白
蛋白质ID	P60709

图片及说明

Fig.1. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Western Blot analysis of 1, hela 2, A549 3, HEPG2 4, mouse brain 5, mouse lung 6, mouse liver 7, rat brain 8, rat lung 9, rat liver cells using primary antibody diluted at 1:1000 (4°C overnight).