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DNA损伤可诱导转录物3多克隆抗体

CHOP Polyclonal Antibody

浏览次数(27) 文献引用(0) 货号(ABP50979)
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商品信息

产品英文名称 CHOP Polyclonal Antibody
免疫原 合成多肽:人源蛋白CHOP,非磷酸化多肽,中心氨基酸为S30.
宿主
反应性 人, 小鼠
应用 ELISA, IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IHC-p:1:100-1:300,IF:1:200-1:1000,ELISA:1:5000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 DDIT3 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. DNA damage inducible transcript 3 functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein, and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
基因ID 1649
别名 DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153
其它 该抗体可检测内源蛋白
蛋白质ID P35638

图片及说明

Fig.1. Western Blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of VEC cells using CHOP Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of VEC cells using CHOP Polyclonal Antibody diluted at 1:500.

Fig.3. Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.

Fig.3. Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.

Fig.4. Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.

Fig.4. Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.

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