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His标签小鼠单克隆抗体(5C3)

Anti-His Tag Mouse Monoclonal Antibody (5C3)

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商品信息

产品英文名称 Anti-His Tag Mouse Monoclonal Antibody (5C3)
免疫原 合成多肽
宿主 小鼠
应用 IF, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:5000,IF:1:1500,IP:1:300。
克隆性 单克隆
亚型 小鼠IgG
纯化工艺 通过使用特异性免疫原的亲和层析,从小鼠腹水中纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
别名 His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

图片及说明

Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.

Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A, transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).

Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A, transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).

本产品已在21种出版物中被引用

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