加入收藏 欢迎光临Abbkine Scientific Co., Ltd官方网站
登录 注册
中文
0

购物车

¥0

NSE 单克隆抗体

NSE Monoclonal Antibody

浏览次数(16) 文献引用(0) 货号(ABM0024)
说明书下载 打印 社交分享 邮件分享
  • 产品概述
  • FAQ
  • 文献引用(0)
  • 用户评论(0)

商品信息

产品英文名称 NSE Monoclonal Antibody
免疫原 合成多肽
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:2000,IF:1:100-1:200,IHC-P:1:200。
克隆性 单克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
分子量 47 KD
储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 ENO2 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme (enolase 2, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
基因ID 2026
别名 ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
其它 该抗体可检测内源蛋白
蛋白质ID P09104

图片及说明

Fig.1. Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates, diluted at 1:3000.

Fig.1. Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates, diluted at 1:3000.

Fig.2. Immunofluorescence analysis of human appendix tissue. 1, NSE Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human appendix tissue. 1, NSE Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse spleen tissue. 1, NSE Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse spleen tissue. 1, NSE Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, NSE Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, NSE Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, NSE Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, NSE Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, NSE Monoclonal Antibody  was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, NSE Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

本产品已在0种出版物中被引用

评价

目前还没有评论

成为第一个评论 “NSE 单克隆抗体” 的人

邮箱地址不会被公开。 必填项已用*标注

39 − = 37

意见反馈
在线咨询
扫码关注

微信公众号

返回顶部
没问题