| 产品英文名称 | ERCC1 Monoclonal Antibody |
| 免疫原 | 合成多肽 |
| 宿主 | 小鼠 |
| 反应性 | 人 |
| 应用 | IHC-P, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:1000，IHC-P：1:100-1:300。 |
| 克隆性 | 单克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从小鼠腹水中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 分子量 | 36 KD |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | The product of ERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. ERCC excision repair 1, endonuclease non-catalytic subunit forms a heterodimer with the XPF endonuclease (also known as ERCC4， and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in ERCC1 result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of ERCC1 may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for ERCC1. The last exon of ERCC1 overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. |
| 基因ID | 2067 |
| 别名 | ERCC1; DNA excision repair protein ERCC-1 |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P07992 |
Fig.1. Western blot analysis of 1) Hela, 2) HepG2, 3) 293T, 4) Jurkat, diluted at 1:2000.
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