| 产品英文名称 | β II tubulin Monoclonal Antibody |
| 免疫原 | 合成多肽 |
| 宿主 | 小鼠 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | IHC-P, IP, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:50000-1:100000，IF：1:100-1:200，IHC-P：1:200，IP：1:200。 |
| 克隆性 | 单克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从小鼠腹水中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. Tubulin beta 2A class IIa encoded by TUBB2A is a beta-tubulin. Defects in TUBB2A are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for TUBB2A. |
| 基因ID | 7280 |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | Q13885 |
| 目标条带分子量（KD) | 50 |
Fig.1. Western blot analysis of 1) mouse brain tissue, 2) rat brain tissue, diluted at 1:100000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, β II tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, β II tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, β II tubulin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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