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β微管蛋白 (HRP偶联) 单克隆抗体

β-tubulin (HRP Conjugated) Monoclonal Antibody

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商品信息

产品英文名称 β-tubulin (HRP Conjugated) Monoclonal Antibody
免疫原 重组蛋白
宿主 小鼠
反应性 人, 仓鼠, 兔, 大鼠, 小鼠, 昆虫, 狗, 猴子, 绵羊, 酵母, 鸡
应用 IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:1000-1:3000,IHC-P:1:50-1:200。
克隆性 单克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 TUBB (tubulin beta class I) encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in TUBB cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for TUBB on chromosomes 1, 6, 7, 8, 9, and 13.
基因ID 203068
其它 该抗体可检测内源蛋白
蛋白质ID P07437
目标条带分子量(KD) 55

图片及说明

Fig.1. Western blot analysis of Hela, with β-tubulin-HRP Conjugated diluted at 1:10000, 20000, 40000, 80000, 160000, 320000.

Fig.1. Western blot analysis of Hela, with β-tubulin-HRP Conjugated diluted at 1:10000, 20000, 40000, 80000, 160000, 320000.

Fig.2. Immunofluorescence analysis of human lung cancer tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody  (red) was diluted at 1:200 (4°C, overnight). 2,  Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human lung cancer tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of Mouse testis tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody  (red) was diluted at 1:200 (4°C, overnight). 2,  Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of Mouse testis tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunofluorescence analysis of rat kidney tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody  (red) was diluted at 1:200 (4°C, overnight). 2,  Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunofluorescence analysis of rat kidney tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2,  Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded Mouse heart tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2,  Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded Mouse heart tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.7. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1,  β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2,  Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.7. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, β-tubulin (HRP Conjugated) Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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