|产品英文名称||ERK1 Mouse Monoclonal Antibody (5E9)|
|免疫原||重组蛋白: ERK1 of MAPK3|
|反应性||人, 大鼠, 小鼠|
|背景||MAPK1 (mitogen-activated protein kinase 1) encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs， act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1.|
Fig.1. Immunofluorescence analysis of rat spleen tissue. 1, ERK1 Mouse Monoclonal Antibody (5E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, ERK1 Mouse Monoclonal Antibody (5E9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.