加入收藏 欢迎光临Abbkine Scientific Co., Ltd官方网站
登录 注册
中文
0

购物车

¥0

胶原蛋白IV小鼠单克隆抗体 (8E5)

Collagen IV Mouse Monoclonal Antibody (8E5)

浏览次数(11) 文献引用(0) 货号(ABM40371)
说明书下载 打印 社交分享 邮件分享
  • 产品概述
  • FAQ
  • 文献引用(0)
  • 用户评论(0)

商品信息

产品英文名称 Collagen IV Mouse Monoclonal Antibody (8E5)
免疫原 合成多肽: Collagen IV
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:IHC-p:1:50-200。
克隆性 单克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从小鼠腹水中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.
基因ID 1282
别名 Collagen alpha-1(IV) chain [Cleaved into: Arresten]
其它 该抗体可检测内源蛋白
蛋白质ID p02462
目标条带分子量(KD) 161

图片及说明

Fig.1. Immunofluorescence analysis of human liver tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.1. Immunofluorescence analysis of human liver tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of mouse spleen tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of mouse spleen tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

本产品已在0种出版物中被引用

评价

目前还没有评论

成为第一个评论 “胶原蛋白IV小鼠单克隆抗体 (8E5)” 的人

邮箱地址不会被公开。 必填项已用*标注

+ 12 = 22

意见反馈
在线咨询
扫码关注

微信公众号

返回顶部
没问题