| 产品英文名称 | Cytokeratin 19 Polyclonal Antibody |
| 免疫原 | 合成多肽：人源蛋白的中央区域， Cytokeratin 19，AA：200-280 |
| 宿主 | 兔 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | ELISA, IF, IHC-P, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IF：1:200-1:1000，IHC-P：1:100-1:300，ELISA：1:10000。 |
| 克隆性 | 多克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 分子量 | 44 KD |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Keratin 19 encoded by KRT19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. |
| 基因ID | 3880 |
| 别名 | KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19 |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P08727 |
Fig.1. Immunofluorescence analysis of human liver tissue. 1, Cytokeratin 19 多克隆 Antibody (red) was diluted at 1:200 (4°,overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.2. Western Blot analysis of various cells using Cytokeratin 19 多克隆 Antibody diluted at 1:1000.
Fig.3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue. 1, CytokeRatin 19 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, CytokeRatin 19 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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