| 产品英文名称 | ERK 1/2 Polyclonal Antibody |
| 免疫原 | 合成多肽：人源蛋白的C末端，ERK 1/2，AA：300-380 |
| 宿主 | 兔 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | ELISA, IF, IHC-P, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IF：1:50-1:200，IHC-P：1:100-1:300，ELISA：1:10000。 |
| 克隆性 | 多克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 分子量 | 42, 44 KD |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Mitogen-activated protein kinase 3 encoded by MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs， act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. |
| 基因ID | 5595 |
| 别名 | MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P27361/P28482 |
Fig.1. Western Blot analysis of various cells using ERK 1/2 多克隆 Antibody diluted at 1:2000.
Fig.2. Immunofluorescence analysis of human lung tissue. 1, ERK 1/2 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunofluorescence analysis of rat lung tissue. 1, ERK 1/2 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, ERK 1/2 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.5. Immunohistochemical analysis of paraffin-embedded mouse lung tissue. 1, ERK 1/2 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, ERK 1/2 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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