Lamin B1多克隆抗体

Lamin B1 Polyclonal Antibody

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商品信息

产品英文名称	Lamin B1 Polyclonal Antibody
免疫原	合成多肽：人源蛋白的N末端，Lamin B1
宿主	兔
反应性	人, 大鼠, 小鼠
应用	ELISA, IF, IHC-P, WB
实验建议	最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IF：1:50-1:200，IHC-P：1:100-1:300，ELISA：1:40000。
克隆性	多克隆
纯化工艺	使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。

商品属性

产品形式	液体溶液
浓度	1 mg/ml
分子量	67 KD
储存缓冲液	PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。
保存建议	自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件	蓝冰运输
警告	本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

附加信息

背景	LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of LMNB1 is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
基因ID	4001
别名	LMNB1; LMN2; LMNB; Lamin-B1
其它	该抗体可检测内源蛋白
蛋白质ID	P20700

图片及说明

Fig.1. Western Blot analysis of various cells using Lamin B1 多克隆 Antibody diluted at 1:2000.

Fig.2. Immunofluorescence analysis of mouse kidney tissue. 1, Lamin B1 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Lamin B1 多克隆 Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, Lamin B1 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue. 1, Lamin B1 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.