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His-tag多克隆抗体

His-tag Polyclonal Antibody

浏览次数(4) 文献引用(0) 货号(ABP50077)
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商品信息

产品英文名称 His-tag Polyclonal Antibody
免疫原 KLH偶联的6X His合成多肽
宿主
应用 ELISA, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:1000-1:3000,ELISA:1:20000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
别名 His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
其它 该抗体可检测内源蛋白
目标条带分子量(KD) 15

图片及说明

Fig.1. Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2) . Antibody was diluted at 1:2000. Secondary Antibody  was diluted at 1:20000.

Fig.1. Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2) . Antibody was diluted at 1:2000. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary Antibody  was diluted at 1:20000.

Fig.2. Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary Antibody was diluted at 1:20000.

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