| 产品英文名称 | Ub (Acetyl Lys29) Polyclonal Antibody |
| 免疫原 | 合成多肽：人源蛋白Ub，乙酰化多肽，中心氨基酸为K29. |
| 宿主 | 兔 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | ELISA, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，ELISA：1:20000。 尚未在其他应用中测试。 |
| 克隆性 | 多克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 储存缓冲液 | 含有50％甘油，0.5％BSA和0.02％叠氮化钠的PBS缓冲液。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27. |
| 别名 | UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion pr |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P62987/P62979/P0CG47/P0CG48 |
| 目标条带分子量（KD) | 80 |
Fig.1. Western Blot analysis of HepG2 cells using Acetyl-Ub (K29) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
Fig.2. Western Blot analysis of HepG2 cells using Acetyl-Ub (K29) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
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