| 产品英文名称 | ATF-2 (phospho Ser112) Polyclonal Antibody |
| 免疫原 | 合成多肽：人源蛋白ATF-2，磷酸化多肽，中心氨基酸为S112. |
| 宿主 | 兔 |
| 反应性 | 人, 大鼠, 小鼠 |
| 应用 | ELISA, IHC-P, IP, WB |
| 实验建议 | 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IHC-p：1:100-1:300。 IP：2-5 ug/mg lysate。 ELISA：1:20000。 尚未在其他应用中测试。 |
| 克隆性 | 多克隆 |
| 纯化工艺 | 使用表位特异性的免疫原，通过亲和层析，从兔抗血清中亲和纯化抗体。 |
| 产品形式 | 液体溶液 |
| 浓度 | 1 mg/ml |
| 储存缓冲液 | 含有50％甘油，0.5％BSA和0.02％叠氮化钠的PBS缓冲液。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | ATF2 encodes a transcription factor (activating transcription factor 2) that is a member of the leucine zipper family of DNA binding proteins. Activating transcription factor 2 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Activating transcription factor 2 binds to the cAMP-responsive element (CRE， an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Activating transcription factor 2 may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this ATF2. |
| 基因ID | 1386 |
| 别名 | ATF2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding pro |
| 其它 | 该抗体可检测内源蛋白 |
| 蛋白质ID | P15336 |
Fig.1. Western Blot analysis of various cells using Phospho-ATF-2 (S112) Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of A549 cells using Phospho-ATF-2 (S112) Polyclonal Antibody diluted at 1:500.
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