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促分裂原活化蛋白激酶激酶1/2 (phospho Ser218/222)多克隆抗体

MEK-1/2 (phospho Ser218/222) Polyclonal Antibody

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商品信息

产品英文名称 MEK-1/2 (phospho Ser218/222) Polyclonal Antibody
免疫原 合成多肽:人源蛋白MEK-1/2,磷酸化多肽,中心氨基酸为S218/222.
宿主
反应性 人, 大鼠, 小鼠
应用 ELISA, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IHC-p:1:100-1:300,ELISA:1:5000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Mitogen-activated protein kinase 1 encoded by MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs, act as an integration point for multiple biochemical signals. Mitogen-activated protein kinase 1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway,Mitogen-activated protein kinase 1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
基因ID 5604/5605
别名 MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein k
其它 该抗体可检测内源蛋白
蛋白质ID Q02750/P36507
目标条带分子量(KD) 48

图片及说明

Fig.1. Western Blot analysis of various cells using Phospho-MEK-1/2 (S218/222) Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Phospho-MEK-1/2 (S218/222) Polyclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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