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线粒体核糖体蛋白S12多克隆抗体

MRP-S12 Polyclonal Antibody

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商品信息

产品英文名称 MRP-S12 Polyclonal Antibody
免疫原 合成多肽:来源于中间区域的人源蛋白MRP-S12. 氨基酸序列为: 40-120
宿主
反应性 人, 小鼠
应用 ELISA, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,ELISA:1:10000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 28S ribosomal protein S12 mitochondrial (mitochondrial ribosomal protein S12) is encoded by nuclear genes MRPS12 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.
基因ID 6183
别名 MRPS12; RPMS12; RPSM12; 28S ribosomal protein S12; mitochondrial; MRP-S12; S12mt; MT-RPS12
其它 该抗体可检测内源蛋白
蛋白质ID O15235

图片及说明

Fig.1. Western Blot analysis of various cells using MRP-S12 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using MRP-S12 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of SH-SY5Y cells using MRP-S12 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of SH-SY5Y cells using MRP-S12 Polyclonal Antibody diluted at 1:1000.

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