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细胞因子信号转导抑制因子1多克隆抗体

SOCS-1 Polyclonal Antibody

浏览次数(12) 文献引用(1) 货号(ABP52477)
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商品信息

产品英文名称 SOCS-1 Polyclonal Antibody
免疫原 合成多肽:来源于中间区域的人源蛋白SOCS-1.
宿主
反应性 人, 大鼠, 小鼠
应用 ELISA, IF, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000。 Immunocytochemistry:1:200-1:1000,ELISA:1:40000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 SOCS1 encodes a member of the STAT-induced STAT inhibitor (SSI, also known as suppressor of cytokine signaling (SOCS, family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO, CSF2/GM-CSF, and interferon (IFN) -gamma. Suppressor of cytokine signaling 1 encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival.
基因ID 8651
别名 SOCS1; SSI1; TIP3; Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3
其它 该抗体可检测内源蛋白
蛋白质ID O15524

图片及说明

Fig.1. Western Blot analysis of various cells using SOCS-1 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using SOCS-1 Polyclonal Antibody diluted at 1:2000.

Fig.2. Immunofluorescence analysis of mouse liver tissue. 1, SOCS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of mouse liver tissue. 1, SOCS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, SOCS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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