|产品英文名称||DR3 Polyclonal Antibody|
|免疫原||合成多肽：来源于中间区域的人源蛋白DR3. 氨基酸序列为： 230-310|
|应用||ELISA, IHC-P, WB|
|实验建议||最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IHC-p：1:100-300 ELISA：1:20000。 尚未在其他应用中测试。|
|背景||Tumor necrosis factor receptor superfamily member 25 encoded by TNFRSF25 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.|
|别名||TNFRSF25; APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; Tumor necrosis factor receptor superfamily member 25; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; Lymphocyte-associated receptor of death; LARD; Protein WSL; Protein WSL-1|
Fig.1. Western Blot analysis of K562 cells using DR3 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.