|产品英文名称||PKC γ Polyclonal Antibody|
|反应性||人, 大鼠, 小鼠|
|应用||ELISA, IHC-P, WB|
|实验建议||最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IHC-p：1:100-300 ELISA：1:20000。 尚未在其他应用中测试。|
|背景||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. Protein kinase C gamma type encoded by PRKCG is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD， specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene.|
|别名||PRKCG; PKCG; Protein kinase C gamma type; PKC-gamma|
Fig.1. Western Blot analysis of A549, THP-1, K562, Mouse brain, Mouse skeletal muscle, Mouse testis cells using PKC γ Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.