|产品英文名称||Cdk4 Polyclonal Antibody|
|反应性||人, 大鼠, 小鼠|
|应用||ELISA, IF, IHC-P, WB|
|实验建议||最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为：WB：1:500-1:2000，IHC-p：1:100-300 ELISA：1:20000。 尚未在其他应用中测试。|
|背景||Cyclin-dependent kinase 4 encoded by CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.|
|别名||CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3|
Fig.1. Western Blot analysis of MCF7 (1， K562 (2).
Fig.2. Immunofluorescence analysis of human stomach tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Cdk4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.4. Immunohistochemical analysis of paraffin-embedded human brain, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded rat muscle, antibody was diluted at 1:100.