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膜联蛋白VII多克隆抗体

Annexin VII Polyclonal Antibody

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商品信息

产品英文名称 Annexin VII Polyclonal Antibody
免疫原 合成多肽:来源于中间区域的人源蛋白Annexin VII.
宿主
反应性 人, 小鼠
应用 ELISA, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IHC-p:1:100-1:300,ELISA:1:20000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2. and 2. kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.
基因ID 310
别名 ANXA7; ANX7; SNX; OK/SW-cl.95; Annexin A7; Annexin VII; Annexin-7; Synexin
其它 该抗体可检测内源蛋白
蛋白质ID P20073

图片及说明

Fig.1. Western Blot analysis of A549 cells using Annexin VII Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of A549 cells using Annexin VII Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

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