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组蛋白H2B多克隆抗体

Histone H2B Polyclonal Antibody

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商品信息

产品英文名称 Histone H2B Polyclonal Antibody
免疫原 合成多肽:来源于中间区域的人源蛋白Histone H2B.
宿主
反应性 人, 大鼠, 小鼠
应用 ELISA, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IHC-p:1:100-1:300,ELISA:1:20000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BD is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3.
基因ID 3017/8339/8343/8344/8346/8347/3017/8345/85236/8340/8342/8341/440689
别名 HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/
其它 该抗体可检测内源蛋白
蛋白质ID P33778/P62807/P58876/Q93079/P06899/O60814/Q99880/Q99879/Q99877/P23527

图片及说明

Fig.1. Western Blot analysis of hela cells using Histone H2B Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of hela cells using Histone H2B Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100.

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