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胰岛素样生长因子结合蛋白3多克隆抗体

IGFBP3 Polyclonal Antibody

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商品信息

产品英文名称 IGFBP3 Polyclonal Antibody
免疫原 合成多肽:来源于中间区域的人源蛋白IGFBP3.
宿主
反应性 人, 大鼠, 小鼠
应用 ELISA, IHC-P, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB:1:500-1:2000,IHC-p:1:100-1:300,ELISA:1:20000。 尚未在其他应用中测试。
克隆性 多克隆
纯化工艺 使用表位特异性的免疫原,通过亲和层析,从兔抗血清中亲和纯化抗体。

商品属性

产品形式 液体溶液
浓度 1 mg/ml
储存缓冲液 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
基因ID 3486
别名 IGFBP3; IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3
其它 该抗体可检测内源蛋白
蛋白质ID P17936

图片及说明

Fig.1. Western Blot analysis of hela, HepG2 cells using IGFBP3 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of hela, HepG2 cells using IGFBP3 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.4. Western Blot analysis of mosue-brian using IGFBP3 Polyclonal Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.4. Western Blot analysis of mosue-brian using IGFBP3 Polyclonal Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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