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组蛋白H3小鼠单克隆抗体(2D10)

Anti-Histone H3 Mouse Monoclonal Antibody (2D10)

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商品信息

产品英文名称 Anti-Histone H3 Mouse Monoclonal Antibody (2D10)
免疫原 重组蛋白
宿主 小鼠
反应性 人, 大鼠, 小鼠, 水稻, 酵母
应用 IF, IHC-P, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB 1:2000-5000,IHC:1:50-300,IF:1:100-500,IP:1:200。
克隆性 单克隆
纯化工艺 通过使用特异性免疫原的亲和层析,从小鼠腹水中纯化抗体。

商品属性

产品形式 液体溶液
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
基因ID 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
别名 HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3
蛋白质ID P68431/Q71DI3/P84243
目标条带分子量(KD) 15

图片及说明

Fig.1. Western blot analysis of 1) Hela, 2) Raw, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.

Fig.1. Western blot analysis of 1) Hela, 2) Raw, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunofluorescence analysis of human liver cancer tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunofluorescence analysis of human liver cancer tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.7. Immunofluorescence analysis of rat liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.7. Immunofluorescence analysis of rat liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.8. Western blot analysis of rice sample, diluted at 1:2000.The first two strips on the left are the concentrated nuclear proteins from the rice sample, and the third lane is the chloroplast proteins from the rice sample.

Fig.8. Western blot analysis of rice sample, diluted at 1:2000.The first two strips on the left are the concentrated nuclear proteins from the rice sample, and the third lane is the chloroplast proteins from the rice sample.

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