加入收藏 欢迎光临Abbkine Scientific Co., Ltd官方网站
登录 注册
中文
0

购物车

¥0

α-Tubulin单克隆抗体(3G5)

Anti-α-Tubulin Monoclonal Antibody (3G5)

浏览次数(244) 文献引用(0) 货号(A01080)
说明书下载 打印 社交分享 邮件分享
  • 产品概述
  • FAQ
  • 文献引用(0)
  • 用户评论(0)

商品信息

产品英文名称 Anti-α-Tubulin Monoclonal Antibody (3G5)
免疫原 重组蛋白
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB 1:5000-10000,IHC:1:50-300,IF:1:200,IP:1:200。
克隆性 单克隆
纯化工艺 通过使用特异性免疫原的亲和层析,从小鼠腹水中纯化抗体。

商品属性

产品形式 液体溶液
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. TUBA1A encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that TUBA1A expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
基因ID 7846/10376
别名 TUBA1A; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain
蛋白质ID Q71U36/P68363
目标条带分子量(KD) 52

图片及说明

Fig.1. Western blot analysis of 1) Hela, 2) rat brian tissue, 3) mouse brain tissue, diluted at 1:5000.

Fig.1. Western blot analysis of 1) Hela, 2) rat brian tissue, 3) mouse brain tissue, diluted at 1:5000.

Fig.2. Immunofluorescence analysis of human colon cancer tissue. 1, α-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human colon cancer tissue. 1, α-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse liver tissue. 1, α-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse liver tissue. 1, α-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, α-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

本产品已在0种出版物中被引用

评价

目前还没有评论

成为第一个评论 “α-Tubulin单克隆抗体(3G5)” 的人

您的电子邮箱地址不会被公开。 必填项已用*标注

7 + 1 =

意见反馈
在线咨询
扫码关注

微信公众号

返回顶部