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核纤层蛋白B1单克隆抗体(15T1)

Anti-Lamin B1 Monoclonal Antibody (15T1)

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商品信息

产品英文名称 Anti-Lamin B1 Monoclonal Antibody (15T1)
免疫原 重组蛋白
宿主 小鼠
反应性 人, 大鼠, 小鼠
应用 IF, IHC-P, IP, WB
实验建议 最佳的工作稀释比例需要客户摸索优化。建议的起始尝试的稀释比为:WB 1:2000-5000,IHC:1:50-300,IF:1:200,IP 1:200。
克隆性 单克隆
纯化工艺 通过使用特异性免疫原的亲和层析,从小鼠腹水中纯化抗体。

商品属性

产品形式 液体溶液
储存缓冲液 PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。
保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
运输条件 蓝冰运输
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of LMNB1 is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
基因ID 4001
别名 LMNB1; LMN2; LMNB; Lamin-B1
蛋白质ID P20700
目标条带分子量(KD) 68

图片及说明

Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.

Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.

Fig.2 Immunofluorescence analysis of human lung cancer tissue. 1, Lamin B1 Monoclonal Antibody (15T1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2 Immunofluorescence analysis of human lung cancer tissue. 1, Lamin B1 Monoclonal Antibody (15T1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Lamin B1 Monoclonal Antibody (15T1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Lamin B1 Monoclonal Antibody (15T1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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