Annexin V-AbFluor™ 488/PI双染细胞凋亡检测试剂盒

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Fig.1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488/PI Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).

Fig.2. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

Fig.3. Hela cells were induced with camptothecin of 4 different concentration(0、1:8000、1:4000、1:2000、1:1000) for 24 hours and stained with Annexin V- AbFluor™ 488 /PI Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

• 该试剂盒检测可以选用哪几个通道？

  建议两个染料分别选用FITC和PI波长相近的通道。

• 该试剂盒做流式细胞术是否要用细胞上清?

  染好之后用细胞混悬液上机即可，不需要用细胞上清。

• 该试剂盒是标记细胞凋亡哪个时期的试剂盒？

  标记细胞凋亡早期。

• Annexin V-FITC细胞凋亡试剂盒是否可以单染？

  可以，只要不使用PI染料就可以了。

• 在加入100ul Annexin V结合缓冲液后，可不可以等到60分钟左右再去做下一步呢?

  因为这个实验是活染，细胞先染完第一种染料，如果间隔时间太长，细胞活力就会受影响。

• 细胞样本较少时，为了防止细胞损失，不用PBS洗涤，可以吗？

  建议至少洗涤2-3次，因为培养基里面的杂质很多，会粘在细胞膜上。洗涤之后充分离心再去除上清液，小心操作，不会有细胞损失的，细胞个数在一个数量级之内都没什么影响。

• 该试剂盒可以用来检测细胞周期吗？

  可以取出其中的PI组分对酒精固定后的细胞进行染色，并用流式细胞仪进行检测和定量。

本产品已在9种出版物中被引用

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