蛋白质定量试剂盒（BCA法）

商品信息

商品属性

附加信息

图片及说明

Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve.

• Reagent A有结晶是正常情况吗？是否影响继续使用？

  结晶主要是温度变化造成的组分析出，为正常情况，可缓慢加热直至完全溶解后正常使用。

• 该试剂盒中的BSA标准品浓度如何控制？

  标准品复溶时，应一次性加入足量标准品稀释液，斡旋10秒钟，静置15分钟，观察是否有沉淀析出，应用加热振摇的方法确保溶解充分。

• 该试剂盒里A和B溶液如何混溶？

  先把A稀释2倍，再按A:B=50:1稀释就行，buffer加的是和待检测样品相同的buffer。

• 该蛋白定量试剂盒范围为50-1000 µg/ml，如果实验的蛋白浓度低于50ug/ml是否可以？有没有做过低于50ug/ml的样品验证

  该试剂盒暂时没有达到这个级别，过低浓度蛋白检测建议客户用增强型的BCA试剂盒。

• 该试剂盒内标准品，溶解之后存在絮状物，应该如何处理？

  建议加入足量的稀释液，用加热振摇的方法使其完全溶解。

本产品已在34种出版物中被引用

• 3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects(KTD3001-10.761)

  作者：Shen M, Wang L, Gao Y, et al. 杂志名称：Materials Today Bio IF：10.761

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• Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model(KTD3001-8.098)

  作者：Ozkan S, Isildar B, Ercin M, et al. 杂志名称：Stem cell research & therapy IF：8.098

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• Construction of a hierarchical micro & nanoporous surface for loading genistein on the composite of polyetheretherketone/tantalum pentoxide possessing antibacterial activity and accelerated osteointegration(KTD3001-7.4)

  作者：S Mei, F Wang, X Hu 杂志名称：Biomater Sci IF：7.4

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• Construction of a hierarchical micro & nanoporous surface for loading genistein on the composite of polyetheretherketone/tantalum pentoxide possessing antibacterial activity and accelerated osteointegration(KTD3001-7.4)

  作者：S Mei, F Wang, X Hu 杂志名称：Biomater Sci IF：7.4

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• Circular RNA circVAPA promotes chemotherapy drug resistance in gastric cancer progression by regulating miR-125b-5p/STAT3 axis(KTD3001-7.1)

  作者：P Deng, M Sun, WY Zhao 杂志名称：World J Gastroenterol IF：7.1

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• Circular RNA TTC3 regulates cerebral ischemia-reperfusion injury and neural stem cells by miR-372-3p/TLR4 axis in cerebral infarction(KTD3001-6.7)

  作者：B Yang, L Zang, J Cui 杂志名称：Stem Cell Research & Therapy IF：6.7

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• FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT/mTOR signaling(KTD3001-6.28)

  作者：Zhao R, Tian L, Zhao B, Sun Y, Cao J, Chen K, Li F, Li M, Shang D, Liu M. 杂志名称：Cell Death Dis IF：6.28

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• VEGFLncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing Gene Transcription Enhancing H3K9 Deacetylation by Recruiting HDAC6(KTD3001-5.201)

  作者：Kai H, Wu Q, Yin R, Tang X, Shi H, Wang T, Zhang M, Pan C 杂志名称：Front Cell Dev Biol IF：5.201

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• LncRNA NORAD promotes vascular endothelial cell injury and atherosclerosis through suppressing VEGF gene transcription via enhancing H3K9(KTD3001-5.201)

  作者：Kai H, Wu Q, Yin R, Tang X, Shi H, Wang T, Zhang M, Pan C 杂志名称：Front Cell Dev Biol IF：5.201

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• Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis(KTD3001-5.2)

  作者：X Lv, Y Lian, Z Liu 杂志名称：Aging IF：5.2

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• Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells(KTD3001-5.115)

  作者：Xie D, Xu C, Ye C, Mei S, Wang L, Zhu Q, Chen Q, Zhao Q, Xu Z, Wei J, Yang L 杂志名称：Int J Nanomedicine IF：5.115

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• Chronic inhibition of chemokine receptor CXCR2 attenuates cardiac remodeling and dysfunction in spontaneously hypertensive rats(KTD3001-4.78)

  作者：YL Zhang, C Geng, J Yang, J Fang, X Yan, PB Li 杂志名称：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2019 IF：4.78

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• Long Non-coding RNA XIST Attenuates Diabetic Peripheral Neuropathy by Inducing Autophagy Through MicroRNA-30d-5p/sirtuin1 Axis(KTD3001-4.188)

  作者：Liu BY, Li L, Bai LW, Xu CS 杂志名称：Front Mol Biosci IF：4.188

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• Graphene Oxide Nanoparticle–Loaded Ginsenoside Rg3 Improves Photodynamic Therapy in Inhibiting Malignant Progression and Stemness of(KTD3001-4.188)

  作者：Lu SL, Wang YH, Liu GF, Wang L, Li Y, Guo ZY, Cheng C 杂志名称：Front Mol Biosci IF：4.188

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• Circular RNA TLK1 Promotes Sepsis-Associated Acute Kidney Injury by Regulating Inflammation and Oxidative Stress Through miR-106a-5p/HMGB1 Axis(KTD3001-4.188)

  作者：HP Xu, XY Ma, C Yang 杂志名称：Frontiers in Molecular Biosciences IF：4.188

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• LncRNA MEG8 promotes NSCLC progression by modulating the miR-15a-5p-miR-15b-5p/PSAT1 axis(KTD3001-4.1)

  作者：K Guo, D Qi, B Huang 杂志名称：Cancer Cell International IF：4.1

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• Circular RNA circVAPA promotes chemotherapy drug resistance in gastric cancer progression by regulating miR-125b-5p/STAT3 axis(KTD3001-3.665)

  作者：Deng P, Sun M, Zhao WY, Hou B, Li K, Zhang T, Gu F 杂志名称：World J Gastroenterol IF：3.665

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• The Local Anesthetic Bupivacaine Inhibits the Progression of Non-Small Cell Lung Cancer by Inducing Autophagy Through Akt/mTOR Signaling(KTD3001-3.5)

  作者：JH Gu, CC Liu, JL Xie 杂志名称：Frontiers in Oncology IF：3.5

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• Exosomal miR-21-5p contributes to ovarian cancer progression by regulating CDK6(KTD3001-3.463)

  作者：Cao J, Zhang Y, Mu J, Yang D, Gu X, Zhang J, 杂志名称：Hum Cell IF：3.463

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• miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia(KTD3001-3.423)

  作者：E Wang, Y Zhang, R Ding 杂志名称：Molecular Medicine Reports IF：3.423

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• Long Noncoding RNA CCDC26 Promotes Thyroid Cancer Malignant Progression via miR-422a/EZH2/Sirt6 Axis(KTD3001-3.337)

  作者：Ma X, Li Y, Song Y, Xu G 杂志名称：Onco Targets Ther IF：3.337

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• Bergamottin Induces DNA Damage and Inhibits Malignant Progression in Melanoma by Modulating miR-145/Cyclin D1 Axis(KTD3001-3.337)

  作者：Zhao Z, Liao N 杂志名称：Onco Targets Ther IF：3.337

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• Exosomal miR-638 Inhibits Hepatocellular Carcinoma Progression by Targeting SP1(KTD3001-3.259)

  作者：Yang, Jing; Li, Bo; Zhao, Shuo; Du, Hongyu; Du, Yaming 杂志名称：Onco Targets Ther IF：3.259

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• Sinigrin attenuates angiotensin II‑induced kidney injury by inactivating nuclear factor‑κB and extracellular signal‑regulated kinase signaling in vivo and in vitro(KTD3001-3.098)

  作者：Cong C, Yuan X, Hu Y, Chen W, Wang Y, Tao L 杂志名称：Int J Mol Med IF：3.098

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• Ginkgolide B protects against cognitive impairment in senescence-accelerated P8 mice by mitigating oxidative stress, inflammation and ferroptosis(KTD3001-2.985)

  作者：Shao L, Dong C, Geng D, He Q, Shi Y, 杂志名称：Biochem Biophys Res Commun IF：2.985

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• Comparison of surface properties, cell behaviors, bone regeneration and osseointegration between nano Tantalum/PEEK composite and nano silicon nitride/PEEK composite(KTD3001-2.69)

  作者：Hu G, Zhu Y, Xu F, Ye J, Guan J, Jiang Y, Di M, Li Z, Guan H, Yao X 杂志名称：J Biomater Sci Polym Ed IF：2.69

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• Biosynthesis of gold nanoparticles using leaf extract of Dittrichia viscosa and in vivo assessment of its anti‑diabetic efficacy(KTD3001-2.664)

  作者：Sanaa Ayyoub · Bahaa Al‑Trad 杂志名称：Drug Delivery and Translational Research IF：2.664

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• Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum(KTD3001-2.36)

  作者：Y Gao, J Xu, C Tang 杂志名称：Parasitology Research IF：2.36

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• Pulsatilla Decoction Combined with 5-Fluorouracil Triggers Immunogenic Cell Death in the Colorectal Cancer Cells(KTD3001-2.314)

  作者：Jie Y, Yang X, Chen W 杂志名称：Cancer Biother Radiopharm IF：2.314

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• miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia(KTD3001-2.1)

  作者：Enbo Wang, Yunyan Zhang, Rongmei Ding 杂志名称：Mol Med Rep IF：2.1

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• Spinosin Attenuates Osteoclastogenesis Through Suppressing NF-κB by the Activation of Nrf2/HO-1 Signaling in Osteoporosis(KTD3001-)

  作者：B Liu, Y Zhang 杂志名称：researchsquare IF：

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• Long non-coding RNA CASC7 Contributes to the Progression of Sepsis-Induced Liver Injury by Targeting miR-217/TLR4 Axis(KTD3001-)

  作者：X Zhou, T Zhang, Y Tang 杂志名称：researchsquare IF：

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