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ExKine™ Pro动物细胞/组织总蛋白提取试剂盒(柱式法)

ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues

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商品信息

产品英文名称 ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues
实验建议 Abbkine的ExKine™ 动物细胞/组织总蛋白提取试剂盒,提供了一种简单、方便的从培养细胞或新鲜组织中抽提总蛋白的方法。

商品属性

试剂盒组分 •Denaturing cell lysis buffer
• Native cell lysis buffer
• Protein extraction filter cartridges
• Collection tubes with cap
• Plastic rods
特点&优势 多用途—适用于新鲜的哺乳动物细胞或组织样品,几乎没有交叉污染。
方便快捷—非变性,有活性的蛋白质可在不到7分钟迅速提取完成。
兼容性好—提取的蛋白适用于多种下游实验,如Western Blot,蛋白分析等。
使用中注意事项 所有实验步骤请在2-8°C下执行。使用预冷的缓冲液和设备,并确保所有溶液都是解冻和均匀的。
保存建议 常温保存12个月。
运输条件 冰袋运输(蓝冰)
警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

附加信息

背景 从动物细胞或组织中制备提取物,提取的蛋白可用于SDS-PAGE、Immunoblottings、IP、ELISA、酶检测以及蛋白质分析和小规模蛋白层析纯化等其他应用。

图片及说明

Fig.1 Protein profiles of mouse liver extracted by denatureing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, Marker; Lane 2, native buffer from A manufacturer; Lane 3, native buffer from Abbkine; Lane 4, denatureing lysis buffer from A manufacturer; Lane 5, denatureing lysis buffer from B manufacturer; Lane 6, denatureing lysis buffer from C manufacturer; Lane 7, denatureing lysis buffer from Abbkine; Lane 8, denatureing lysis buffer from D manufacturer.

Fig.1 Protein profiles of mouse liver extracted by denatureing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, Marker; Lane 2, native buffer from A manufacturer; Lane 3, native buffer from Abbkine; Lane 4, denatureing lysis buffer from A manufacturer; Lane 5, denatureing lysis buffer from B manufacturer; Lane 6, denatureing lysis buffer from C manufacturer; Lane 7, denatureing lysis buffer from Abbkine; Lane 8, denatureing lysis buffer from D manufacturer.

Fig.2 Total proteins were extracted from 293 cells and mouse liver. Lane 1, 5 μL proteins extracted by B assay kits; Lane 2, 2.5 μL proteins extracted by B assay kits; Lane 3, 5 μL proteins extracted by Abbkine assay kits; Lane 4, 2.5 μL proteins extracted by Abbkine assay kits.

Fig.2 Total proteins were extracted from 293 cells and mouse liver. Lane 1, 5 μL proteins extracted by B assay kits; Lane 2, 2.5 μL proteins extracted by B assay kits; Lane 3, 5 μL proteins extracted by Abbkine assay kits; Lane 4, 2.5 μL proteins extracted by Abbkine assay kits.

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