| 产品英文名称 | IPKine™ Anti-HA Magnetic IP Kit |
| 产品中文名称 | IPKine™ HA标签蛋白免疫沉淀试剂盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 样本类型 | IP |
| 试剂盒组分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-HA Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer HA Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| 特点&优势 | • 高效：特异性强、靶蛋白结合量高，≥0.6 mg HA标签融合蛋白/mL磁珠； • 便捷：可结合多种形式的HA标签蛋白（N端HA融合蛋白、C端HA融合蛋白）； • 通用：提供IP实验所需的所有必要缓冲液； • 可靠：提供阴性对照，可排除IgG本身和目的蛋白或其它特定生物分子的非特异性结合； • 灵活：本试剂盒提供三种洗脱方法（HA多肽竞争洗脱、酸洗脱和SDS-PAGE Loading Buffer洗脱方法）。 |
| 保存建议 | 按各组分标签提示分开存储，保质期12个月。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Figure. The immunoprecipitation effect of Anti-HA Magnetic IP Kit used for HA-Tag fusion protein. HEK293T cells were transfected with HA-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Elution Buffer; Lane 5 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Working HA Peptide. By using peptide elution and acid elution, only contained HA-Tag fusion protein, did not contain heavy and light chains of antibody.
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