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Fig. Hela cells stained with Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1). A: Red fluorescence indicates healthy mitochondria, B: Green fluorescence indicates mitochondria in poor health (30min incubation in 20uM CCCP).

• 该试剂盒能用分光光度计检测吗？

  该试剂盒运用的是荧光定量法，不建议用分光光度计检测。

• 如何在保证小老鼠精子的体外存活的前提下，用该试剂盒进行线粒体膜电位分析？

  用HBSS溶液培养小鼠精子，并用该缓冲液配置染色工作液，进行活细胞染色。

• 该试剂盒可以用分光光度计检测吗？

  不建议用，该试剂盒是基于线粒体跨膜电位的变化，运用JC-1进行细胞染色，区分健康和凋亡细胞，建议用流式细胞仪或者荧光显微镜进行检测。

• 该试剂盒如果储存时间偏长染液吸出是无色，这应该如何处理？

  该试剂盒如果客户低温储存时间偏长，染料可能会低温析出，建议将瓶子振摇，然后水浴37度加热，混匀即可。

本产品已在(16)种出版物中被引用

• Growth hormone protects against ovarian granulosa cell apoptosis: Alleviation oxidative stress and enhancement mitochondrial function

  作者：Wang J, Wu J, Zhang Y, Zhang J, Xu W, Wu C, Zhou P, 杂志名称：Reprod Biol IF：2

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• Mitochondrial Ca2+ overload due to altered proteostasis amplifies apoptosis in C2C12 myoblasts under hypoxia: Protective role of nanocurcumin formulation

  作者：AD Kushwaha, N Kalra, R Varshney, D Saraswat 杂志名称：IUBMB life IF：4

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• Comparison of IVF and IVM outcomes in the same patient treated with a modified IVM protocol along with an oocytes-maturing system containing melatonin: A pilot study

  作者：X Li, Y Mu, N Elshewy 杂志名称：Life Sciences IF：4

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• Glyoxal Damages Human Aortic Endothelial Cells by Perturbing the Glutathione, Mitochondrial Membrane Potential, and Mitogen-Activated Protein Kinase

  作者： 杂志名称：BMC Cardiovascular Disorders IF：2

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• Mitochondrial Ca2+ overload due to altered proteostasis amplifies apoptosis in C2C12 myoblasts under hypoxia: Protective role of nanocurcumin formulation

  作者：Kushwaha, Asha D., et al. 杂志名称：IUBMB life IF：5

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• Evaluation of Potential Neuroprotective Effects of Vanillin Against MPP+/MPTP-Induced Dysregulation of Dopaminergic Regulatory Mechanisms in SH-SY5Y Cells and a Mouse Model of Parkinson’s Disease

  作者：Rani, Linchi, et al. 杂志名称：Molecular Neurobiology IF：5

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• Evaluation of Potential Neuroprotective Effects of Vanillin Against MPP+/MPTP-Induced Dysregulation of Dopaminergic Regulatory Mechanisms in SH-SY5Y Cells and a Mouse Model of Parkinson’s Disease

  作者：Rani, Linchi, et al. 杂志名称：Molecular Neurobiology IF：5

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• The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells

  作者：ZL Li, J Mi, L Lu 杂志名称：Food & Function IF：6

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• Evaluation of Potential Neuroprotective Effects of Vanillin Against MPP+/MPTP-Induced Dysregulation of Dopaminergic Regulatory Mechanisms in SH-SY5Y Cells …

  作者：L Rani, B Ghosh, MH Ahmad, AC Mondal 杂志名称：Molecular Neurobiology IF：5

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• miR‐139‐5p upregulation alleviated spontaneous recurrent epileptiform discharge‐induced oxidative stress and apoptosis in rat hippocampal neurons via regulating

  作者：C Zhao, F Yang, X Wei, J Zhang 杂志名称：Cell Biology International IF：3

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• Evaluation of Potential Neuroprotective Effects of Vanillin Against MPP+/MPTP-Induced Dysregulation of Dopaminergic Regulatory Mechanisms in SH-SY5Y Cells …

  作者：L Rani, B Ghosh, MH Ahmad, AC Mondal 杂志名称：Molecular Neurobiology IF：5

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• Mitochondrial Ca2+ overload due to altered proteostasis amplifies apoptosis in C2C12 myoblasts under hypoxia: Protective role of nanocurcumin formulation

  作者：Kushwaha, Asha D., et al. 杂志名称：IUBMB life IF：5

  查看文献

• Smurf1 restricts the antiviral function mediated by USP25 through promoting its ubiquitination and degradation

  作者：Qian G, Hu X, Li G, et al 杂志名称：Biochem Biophys Res Commun IF：3

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• Mitochondrial Ca2+ overload due to altered proteostasis amplifies apoptosis in C2C12 myoblasts under hypoxia: Protective role of nanocurcumin formulation

  作者：AD Kushwaha, N Kalra, R Varshney, D Saraswat 杂志名称：IUBMB life IF：4

  查看文献

• miR-429 suppresses tumor migration and invasion by targeting CRKL in hepatocellular carcinoma via inhibiting Raf/MEK/ERK pathway and epithelial-mesenchymal transition

  作者：Guo C M, Zhao D T, Zhang Q L, et al 杂志名称：Scientific Reports IF：4

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• Glyoxal Damages Human Aortic Endothelial Cells by Perturbing the Glutathione, Mitochondrial Membrane Potential, and Mitogen-Activated Protein Kinase Pathways

  作者：MZ Xie, C Guo, JQ Dong 杂志名称：researchsquare IF：

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