- 中文产品名称
- 重组人转铁蛋白(TF)
- 英文产品名称
- Human Transferrin protein
- 种属
- 人
- 表达宿主
- 人类胚胎肾细胞
- 分子
- TF
- 活性
- 检测进行中
- 蛋白长度
- 重组人转铁蛋白由690个氨基酸组成,预测分子量为76.6 kDa。 在还原条件下,SDS-PAGE中人转铁蛋白的表观分子量约为70-100 kDa。
- 纯度
- ≥ 95 % ,使用SDS-PAGE检测
- 产品形式
- 冻干粉,冻干缓冲液为无菌的 PBS,pH 7.4。
- 特点
- 采用 LAL 法检测,每 1 μg 蛋白质中的 EU 含量小于 1.0。
- 分子量
- 76.6 kDa
- 使用中注意事项
- 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
- 保存建议
- 本产品为冻干粉形式,建议:将冻干粉的重组人转铁蛋白(TF)产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。
- 运输条件
- 常温运输
- 警告
- 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。
- 背景
- Transferrin (Tf),is an iron transporter, which is mainly biosynthesized in the liver, and it can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc.The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. Research indicates that brain-type Tf levels is decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production.
- 基因ID
- 7018
- 蛋白质ID
- P02787