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Fig. Standard Curve of Lactate assay. The y-axis is OD450 and the x-axis is Lactate concentration (mM).

• 如果客户样本不能在同一天收集检测，会间隔几天，该样品是Lactate Assay Buffer处理过后冻在-80度吗？

  可以。

• 乳酸检测试剂盒可以检测细胞培养液里的乳酸含量吗？

  可以，但是需要把细胞给分离出来，单独取定量的细胞培养液进行检测。

• 该试剂盒是否可以测乳酸生成？

  可以测，建议分别取生化反应前后的样本，并分别进行检测，计算乳酸含量变化的差值。

• 该试剂盒里面有一个操作是需要去除所有蛋白质，该需要怎么做？

  需要，用过滤除杂的方法，内源性乳酸脱氢酶（LDH）可降解乳酸。含有LDH （如培养基或组织裂解液）的样品应通过10 kDa MW旋转过滤器过滤，以去除所有蛋白质，然后-80°C 保存。

• 该试剂盒检测细胞培养液的前处理的方法是什么？

  以2000g的速度离心5min，取0.5-10 µL上清液可直接用Lactate Assay Buffer稀释到50 µL/孔进行检测。

• 本试剂盒中检测OD0 min的意义是什么？设置OD30 min-OD0 min的作用是什么？

  本试剂盒的OD0 min是指样本溶液中可能存在背景OD值，OD30 min-OD 0min的作用是将检测结果减去背景OD值，消除实验背景。

• 该乳酸检测试剂盒加完buffer之后是颠倒混匀，还是要用移液器吹打混匀呢？

  离心管里面要用斡旋仪斡旋10s。

本产品已在(17)种出版物中被引用

• The potential role of CMC1 as an immunometabolic checkpoint in T cell immunity.

  作者：Chen, Yuwen, et al. 杂志名称： Oncoimmunology  IF：6

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• CXCR4 regulates macrophage M1 polarization by altering glycolysis to promote prostate fibrosis.

  作者：Zhang, Yi, et al. 杂志名称： Cell Communication and Signaling  IF：8

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• Targeting the PDK/PDH axis to reverse metabolic abnormalities by structure-based virtual screening with in vitro and in vivo experiments.

  作者：Yue, Jianda, et al. 杂志名称：International Journal of Biological Macromolecules  IF：8

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• Enhancing photocatalytic hydrogen production from engineered Escherichia coli-biohybrid system via intracellular electron redirection.

  作者：Lin, Song, et al. 杂志名称：Chemical Engineering Journal  IF：13

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• Hypoxia promotes histone H3K9 lactylation to enhance LAMC2 transcription in esophageal squamous cell carcinoma.

  作者：Zang, Yong, et al. 杂志名称：Iscience IF：5

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• Via inhibiting glycolysis and oxidative respiration and promoting ferroptosis, ETFDH inhibits the proliferation of colorectal cancer.

  作者：Feng, Yaoyao, et al. 杂志名称： IF：

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• Divalent Metal Cations Favor Bone Regeneration Through Promoting Aerobic Glycolysis of Differentiated Mscs and Modulating Activity of Sensory and Autonomic Nervous Systems.

  作者：Luo, Ying, et al. 杂志名称： IF：

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• Oncogenic HPV-induced high expression of ESM1 predicts poor prognosis and regulates aerobic glycolysis in cervical cancer."

  作者：Yuan, Lin, et al. 杂志名称：Iscience IF：5

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• Remote ischemic postconditioning-mediated neuroprotection against stroke by promoting ketone body-induced ferroptosis inhibition.

  作者：Huang, Lin-yan, et al. 杂志名称：ACS chemical neuroscience IF：4

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• Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma.

  作者：Wang, Yinghao, et al. 杂志名称：Iscience  IF：5

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• TAB182 regulates glycolytic metabolism by controlling LDHA transcription to impact tumor radiosensitivity.

  作者：Chen, Shi, et al. 杂志名称：Cell Death & Disease  IF：8

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• Natural product fargesin interferes with H3 histone lactylation via targeting PKM2 to inhibit non‐small cell lung cancer tumorigenesis.

  作者：Guo, Zizhang, et al. 杂志名称： BioFactors  IF：5

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• Regulatory effect of lactate on peripheral blood CD4+ T cell subsets in patients with rheumatoid arthritis." Beijing da xue xue bao. Yi xue ban= Journal of Peking University.

  作者：Huang, Huina, et al. 杂志名称：Health Sciences IF：2

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• Lactate administration induces skeletal muscle synthesis by influencing Akt/mTOR and MuRF1 in non‐trained mice but not in trained mice.

  作者：Kyun, Sunghwan, et al. 杂志名称： Physiological Reports  IF：2

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• TNF‐α‐Induced KAT2A Impedes BMMSC Quiescence by Mediating Succinylation of the Mitophagy‐Related Protein VCP.

  作者：Su, Zepeng, et al. 杂志名称：Advanced Science  IF：14

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• Lactate Facilitates Pancreatic Repair Following Acute Pancreatitis by Promoting Reparative Macrophage Polarization.

  作者：Jiang, Jing, et al. 杂志名称： IF：

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• KAT7 enhances the proliferation and metastasis of head and neck squamous carcinoma by promoting the acetylation level of LDHA.

  作者：Lu, Ying, et al. 杂志名称：Cancer Letters IF：9

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