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Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve.

• 该试剂盒里A和B溶液如何混溶？

  先把A稀释2倍，再按A:B=50:1稀释就行，buffer加的是和待检测样品相同的buffer。

• 该试剂盒中的BSA标准品浓度如何控制？

  标准品复溶时，应一次性加入足量标准品稀释液，斡旋10秒钟，静置15分钟，观察是否有沉淀析出，应用加热振摇的方法确保溶解充分。

• 该试剂盒内标准品，溶解之后存在絮状物，应该如何处理？

  建议加入足量的稀释液，用加热振摇的方法使其完全溶解。

• 该蛋白定量试剂盒范围为50-1000 µg/ml，如果实验的蛋白浓度低于50ug/ml是否可以？有没有做过低于50ug/ml的样品验证

  该试剂盒暂时没有达到这个级别，过低浓度蛋白检测建议客户用增强型的BCA试剂盒。

本产品已在(20)种出版物中被引用

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